Analytical standardization of Piccha basti using Physicochemical and Phytochemical Parameters.

Background: The standardization of Piccha Basti (therapeutic enema procedure) used in ulcerative colitis involves integrating organoleptic, physicochemical and phytochemical analysis to ensure its consistency, safety, and effectiveness. By employing modern analytical methods, it becomes possible to optimize quality control, ensuring that each batch of Piccha basti maintains the same therapeutic value and is free from contaminants. Method: The standardization of Piccha basti was conducted using three samples prepared in different time with same method and analyzed to ensure standard values along with quality and consistency. Organoleptic evaluation assessed sensory characteristics such as color, odor, taste, appearance, and texture. Result: Physicochemical analysis revealed that the pH levels were near to neutral lies in between 6.05 to 6.24. Specific Gravity values ranged from 1.047 to 1.072, whereas viscosity ranges between 276 cP (centipoise) – 380.6 cP. The Refractive Index was consistent at 1.290 – 1.362, reflecting uniform optical properties. The total solid content varied slightly, from 1.577 gm. to 1.667 gm. and total ash 5.383 % to 6.024 %. Acid insoluble ash 1.135 % to 1.794 % whereas water soluble ash varies from 4.166 % to 4.771 %, water soluble extract varies from 47.514 % to 50.741 % whereas acid soluble extract 46.783 % to 56.4 %. Centrifugation test varies from 46.2 sec to 50.26 sec and creaming test all shows upward movements. Phytochemical analysis provided for each sample, confirming the presence of essential phytochemical compounds. Conclusion: The results demonstrated a high degree of uniformity across all samples, ensuring reproducibility and reliability in the formulation of Piccha Basti. These findings support the standard values for reference, therapeutic use of the standardized formulation in clinical settings.