A useful architecture based on layer by layer self-assembled monolayers for the determination of receptor for activated c kinase 1 with a portable potentiostat

A layer by layer self-assembled monolayer-based (SAMs) portable immunosensor was developed using screen printed carbon electrodes (SPCEs) for the sensitive and selective determination of receptor for activated C kinase 1 (RACK-1), which has a remarkable protein among cancer biomarkers. Gold nanoparticles (AuNPs) conjugated with 4-aminothiophenol (4-ATP) and (3-Aminopropyl)triethoxysilane were used in the design of the biosensor. The biorecognition element generated by powerful materials can determine the target molecule RACK-1 in the range of 0.002–0.8 ng/mL associated with a limit of detection of 0.001 ng/mL. The highlight of the study is that the analytical characteristics of the biosensor, such as the determination range, storage life, selectivity, and reproducibility, can be monitored with a portable potentiostat. The current biosensor design, which is developed based on layer by layer SAMs and shows high selectivity in the presence of different proteins and in artificial serum, is promising for further studies with human serum.