Synthesis and biological evaluation of some new tetrazole derivatives by using oxazine and thiazine compounds

This research included, in its first part, the preparation of the chalcone compound (M) from the reaction of para-nitroacetophenone with benzaldehyde in a basic medium of 40% aqueous sodium hydroxide solution. The second part of the research included the conversion of the compound (M) into the two compounds derived from 2-amino Thiazine (MS) and 2-aminooxazine (MO) by reacting the compound (M) with thiourea or urea, respectively, in a medium of alcoholic sodium hydroxide. In the third part of the research, the amine group in the two compounds (MS) and (MO) was exploited to prepare Schiff bases (MS1-4) and (MO1-4) through the reaction of the two compounds (MS) and ((MO) with some benzaldehyde derivatives. In the last part of the research, the tetrazole compounds (MST1-4) and (MOT1-4) were prepared From the reaction of the prepared Schiff bases (MS1-4) and (MO1-4) with sodium azide in a medium of 1,4-dioxane, a test was conducted for the biological effectiveness of the prepared tetrazole compounds against Gram-positive and Gram-negative bacteria. In addition, some spectroscopic and physical measurements were performed of the prepared compounds.