Expression Profiles of lncRNA H19 and miR-29b-3p in Prostate Cancer Cell Lines

<strong>Objective:</strong> We aimed to evaluate the expression levels of long non-coding RNA H19 and microRNA-29b-3p (miR-29b-3p) in human prostate cancer cell lines (PC3, DU145, LNCaP) and in normal prostate epithelial cells (PNT1A) to explore their potential roles and interactions in prostate cancer biology. <strong>Materials and Methods: </strong>All cell lines PC3, DU145, LNCaP, and PNT1A were cultured in the same type of medium, RPMI-1640. Total RNA was extracted from the cells once they reached confluency. H19 and miR-29b-3p levels were measured by real-time quantitative PCR and normalised to GAPDH and U6 snRNA, respectively. Their relative level was determined by the 2-ΔΔCt method. <strong>Results:</strong> The alterations in lncRNA H19 and miR-29b-3p levels were statistically significant across all cancer cell lines compared to PNT1A cells. H19 levels were notably higher in PC3, moderately increased in DU145, and significantly decreased in LNCaP. Conversely, miR-29b-3p levels increased in DU145 and LNCaP but decreased markedly in PC3. Statistical analysis particularly showed significant differences between PC3 and other cell lines for lncRNA H19 and between PC3 and DU145 for miR-29b-3p (p&lt;0.05). <strong>Conclusion:</strong> Our findings show an inverse relationship between H19 and miR-29b-3p in PC3 cells, indicating that H19 may function as a molecular sponge for miR-29b-3p. The differences we observed in the expression levels between LNCaP and the others show that the H19–miR-29b-3p axis may be affected by androgen receptor signalling and tumour aggressiveness. To better understand the underlying mechanism and its potential clinical relevance, additional functional studies are necessary.