Rapid <scp>DNA</scp> / <scp>eDNA</scp> ‐Based <scp>ID</scp> Tools for Improved Chondrichthyan Monitoring and Management

Rapid DNA/eDNA-based ID tools, which detect specific genetic patterns without requiring sequencing, are essential for biodiversity and wildlife trade monitoring, particularly for species of conservation concern. However, the practical application of these methods remains limited by the availability of standardised protocols, accessibility of resources, and coverage across diverse taxa. This challenge is especially pronounced for Chondrichthyes, a group heavily overexploited due to fishing and illegal trade, and with data scarcity for conservation assessments. Despite their ecological and economic importance, many species lack reference sequences in databases, as well as other molecular data and tools, hindering the development of molecular tools for species identification and trade regulation. This review synthesises the current state of rapid DNA/eDNA-based ID tools for the detection of chondrichthyan species, including established and emerging methods. It also compiles available taxon-specific primers to facilitate efficient species identification and recommends the most suitable methods. We identify key gaps in taxonomic and geographic coverage, emphasising the need for further research to expand these tools to under-represented species and regions. Additionally, we highlight the importance of integrating genetic approaches into enforcement frameworks to enhance conservation strategies and regulatory compliance. By providing an accessible reference for time- and cost-effective genetic monitoring, this work will support evidence-based decision-making and improve the practical application of rapid DNA/eDNA-based ID tools in the conservation and management of Chondrichthyes species worldwide.

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Publication Details

Journal
Molecular Ecology Resources
Published
2025-09-19
DOI
https://doi.org/10.1111/1755-0998.70044
Citations
3
Primary Topic
Identification and Quantification in Food
Type
article
Field-Weighted Citation Impact
2.42
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article

Rapid DNA / eDNA ‐Based ID Tools for Improved Chondrichthyan Monitoring and Management

Cíntia Povill, Leonardo Manir Feitosa, Yan Torres, Vanessa Paes da Cruz et al.
3 citations
Molecular Ecology Resources
Identification and Quantification in Food
2.42
article

Rapid DNA / eDNA ‐Based ID Tools for Improved Chondrichthyan Monitoring and Management

Cíntia Povill, Leonardo Manir Feitosa, Yan Torres, Vanessa Paes da Cruz, Ingrid Vasconcellos Bunholi, Mário G. Lopes, Marcelo Merten Cruz, E. Jesus, Marcela Alvarenga, Rodrigo Rodrigues Domingues, Antônio M. Solé‐Cava, Patrícia Charvet, Aisni Mayumi Correia de Lima Adachi, Daniel M. Souza
article en
3 citations

Abstract

Rapid DNA/eDNA-based ID tools, which detect specific genetic patterns without requiring sequencing, are essential for biodiversity and wildlife trade monitoring, particularly for species of conservation concern. However, the practical application of these methods remains limited by the availability of standardised protocols, accessibility of resources, and coverage across diverse taxa. This challenge is especially pronounced for Chondrichthyes, a group heavily overexploited due to fishing and illegal trade, and with data scarcity for conservation assessments. Despite their ecological and economic importance, many species lack reference sequences in databases, as well as other molecular data and tools, hindering the development of molecular tools for species identification and trade regulation. This review synthesises the current state of rapid DNA/eDNA-based ID tools for the detection of chondrichthyan species, including established and emerging methods. It also compiles available taxon-specific primers to facilitate efficient species identification and recommends the most suitable methods. We identify key gaps in taxonomic and geographic coverage, emphasising the need for further research to expand these tools to under-represented species and regions. Additionally, we highlight the importance of integrating genetic approaches into enforcement frameworks to enhance conservation strategies and regulatory compliance. By providing an accessible reference for time- and cost-effective genetic monitoring, this work will support evidence-based decision-making and improve the practical application of rapid DNA/eDNA-based ID tools in the conservation and management of Chondrichthyes species worldwide.

Molecular Ecology ResourcesVol. 25(8)
Universidade Federal do Rio de Janeiro (BR), University of California, Santa Barbara (US), Universidade do Porto (PT), University of Basel (CH), Universidade Federal do Ceará (BR), Instituto Español de Oceanografía (ES), Instituto de Desenvolvimento Sustentável Mamirauá (BR), Universidade Federal do Pará (BR), The University of Texas at Austin (US), Universidade Estadual Paulista (Unesp) (BR)
Openalex Percentile: Top 10%
Identification and Quantification in Food
2.42
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