Surface Sterilization Soybean Nodules for Microbiological Studies v1

This protocol describes thesurface sterilization and storage of root nodulesfor microbiological research, ensuring the removal ofexternal contaminantswhile maintaining viable internal microbial communities. The process includes asaline wash to remove debris,bleach sterilization, andglycerol stock preparationfor storage. The sterilized nodules are plated onLBA mediato verify sterility before downstream applications.