Glycerol Stock Solution Preparation for Long-Term Bacterial Storage v1
This protocol describes thepreparation of glycerol stock solutionsforlong-term bacterial preservationat-70°C or -80°C. Using acryoprotectant (glycerol)prevents cell damage due to ice crystal formation. The process includesbacterial culture growth, centrifugation, mixing with glycerol, and freezingfor long-term viability.
Authors
- J. D. Eisenback (ORCID: https://orcid.org/0000-0002-2102-4867)
- Mohammad Ali (ORCID: https://orcid.org/0000-0002-9404-9301)
- Fatima Tuz Zohora Mony
Institutions
- Virginia Tech (US)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.17504/protocols.io.j8nlkdb91g5r/v1
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00