PCR Run Protocol for Bacterial 16S rRNA Gene Amplification v1
This protocol describes thepolymerase chain reaction (PCR) setup and amplificationof bacterial16S rRNA genesusing27F and 1492R primers. The method utilizesPromega GoTaq® Green Master Mix (2x)and follows a standardthermal cycling programoptimized for bacterial DNA. Propersterile techniques, contamination prevention, and accurate pipettingare crucial to obtaining high-quality amplification results. The protocol includesnegative controlsto ensure reaction validity.
Authors
- J. D. Eisenback (ORCID: https://orcid.org/0000-0002-2102-4867)
- Mohammad Ali (ORCID: https://orcid.org/0000-0002-9404-9301)
- Fatima Tuz Zohora Mony
Institutions
- Virginia Tech (US)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.17504/protocols.io.eq2lyxz8qgx9/v1
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00