Agarose Gel Electrophoresis for DNA Analysis v1
This protocol describes thepreparation and execution of agarose gel electrophoresisfor DNA analysis. The method is used toseparate DNA fragments based on size, using anelectric field in an agarose gel matrix. The gel is stained withethidium bromide (EtBr)for visualization underUV light. This protocol followsstrict safety guidelinesfor handlingEtBr, a known mutagen. Properpersonal protective equipment (PPE), designated waste disposal, and workspace decontaminationare required.
Authors
- J. D. Eisenback (ORCID: https://orcid.org/0000-0002-2102-4867)
- Mortazavi Seyed Ali
- Fatima Tuz Zohora Mony
Institutions
- Virginia Tech (US)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.17504/protocols.io.261geepeyg47/v1
- Primary Topic
- Microbial Community Ecology and Physiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00