Aurantivibrio plasticivorans gen. nov., sp. nov. and Aurantivibrio infirmus sp. nov., biodegradable plastic-degrading bacteria belonging to the family Cellvibrionaceae, isolated from the coast of Japan
Two Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterial strains, designated 4NK60-0013<sup>T</sup> and 4NH20-0068<sup>T</sup>, were isolated from biodegradable plastic films immersed in the coastal waters of Japan. These strains showed the ability to degrade biodegradable plastics, poly(butylene succinate-<i>co</i>-adipate) (PBSA) and poly(<i>ε</i>-caprolactone). Phylogenetic analyses based on the 16S rRNA gene sequences revealed that strains 4NK60-0013<sup>T</sup> and 4NH20-0068<sup>T</sup> belong to the family <i>Cellvibrionaceae</i> in the order <i>Cellvibrionales</i> and are closely related to members of the genus <i>Gilvimarinus</i>. The species most closely related to strains 4NK60-0013<sup>T</sup> and 4NH20-0068<sup>T</sup> were <i>Gilvimarinus agarilyticus</i> M5c<sup>T</sup> and <i>Gilvimarinus polysaccharolyticus</i> YN3<sup>T</sup>, with 16S rRNA gene sequence similarities of 94.4 and 93.3%, respectively. The sequence similarity between the two novel strains was 94.8%, indicating that they represented separate species. The genome length and G+C content of the genome sequence of strain 4NK60-0013<sup>T</sup> were 4,354,061 bp and 46.9 mol%, respectively, whereas those of strain 4NH20-0068<sup>T</sup> were 4,154,637 bp and 43.3 mol%, respectively. The average nucleotide identity values between the strains were <72.0%, similar to the values between the strains and type strains of species from the genera <i>Cellvibrio</i>, <i>Gilvimarinus</i> and <i>Marinimicrobium</i>. Based on phenotypic, cellular fatty acid and phylogenetic characteristics, the two isolates represent novel species within a new genus of the family <i>Cellvibrionaceae</i>, for which the names <i>Aurantivibrio plasticivorans</i> gen. nov., sp. nov. (type species) and <i>Aurantivibrio infirmus</i> sp. nov. have been proposed. Additionally, these strains and other bacteria belonging to the family <i>Cellvibrionaceae</i> have been shown to biodegrade PBSA, a biodegradable plastic. The type strains of <i>A. plasticivorans</i> and <i>A. infirmus</i> are 4NK60-0013<sup>T</sup> (=NBRC 116180<sup>T</sup>=TBRC 19146<sup>T</sup>) and 4NH20-0068<sup>T</sup> (=NBRC 116181<sup>T</sup>=TBRC 19147<sup>T</sup>), respectively.
Authors
- Kohei Hidaka (ORCID: https://orcid.org/0000-0002-3883-2246)
- Dai‐ichiro Kato (ORCID: https://orcid.org/0000-0002-8255-8790)
- Tokichi Miyakawa
- Shun’ichi Ishii (ORCID: https://orcid.org/0000-0002-0203-8569)
- Rieko Kasaishi
- Risa Yokoyama
- Yoko Furuno
- Kazuya Fukuda (ORCID: https://orcid.org/0000-0002-4355-1545)
- Asuka Arimoto (ORCID: https://orcid.org/0000-0001-8220-5920)
- Mamiko Shimamura
- Yoshiyuki Ishitani (ORCID: https://orcid.org/0000-0001-8941-6990)
- Kei Kamino (ORCID: https://orcid.org/0009-0009-8353-3841)
- Kunifumi Tagawa (ORCID: https://orcid.org/0000-0001-5634-9373)
- Tatsuya Ueki (ORCID: https://orcid.org/0000-0002-3112-9507)
- Moriyuki Hamada (ORCID: https://orcid.org/0000-0001-8239-6296)
- Takamasa Miura
- Yoko Kusuya (ORCID: https://orcid.org/0000-0002-5724-487X)
- Y. Uchino (ORCID: https://orcid.org/0000-0003-3467-4217)
Institutions
- Kagoshima University (JP)
- Hiroshima University (JP)
- Japan Agency for Marine-Earth Science and Technology (JP)
- National Institute of Technology and Evaluation (JP)
- Kitasato University (JP)
Publication Details
- Journal
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Published
- 2025-09-19
- DOI
- https://doi.org/10.1099/ijsem.0.006920
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00