Optimized Protocol for Primary Rat Hepatocyte Isolation and a Model for Investigating Experimental Steatosis
The current protocol presents an efficient and highly reproducible method for isolating primary rat hepatocytes in high yield with high viability. Additionally, the protocol provides a foundation for studying the pathophysiology of fatty liver disease.
Authors
- Shtaywy Abdalla (ORCID: https://orcid.org/0000-0003-4532-276X)
- Mohammad Alsalem (ORCID: https://orcid.org/0000-0001-5575-4377)
- Amani A. Harb (ORCID: https://orcid.org/0000-0001-9408-533X)
Institutions
- Al-Ahliyya Amman University (JO)
- University of Jordan (JO)
Publication Details
- Journal
- Methods and Protocols
- Published
- 2025-09-19
- DOI
- https://doi.org/10.3390/mps8050111
- Primary Topic
- Liver Disease Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00