Highly transparent elastic optical microcavities for interferometric mapping of cell mechanics

Elastic resonator interference stress microscopy (ERISM) enables label-free, non-invasive measurements of cellular forces by detecting the cell-induced deformation of an optical microcavity using optical interference. Here, we present an improved microcavity design that utilizes a high-refractive-index elastomer, eliminating the need for the top metal layer required in traditional ERISM microcavities. This simplifies the fabrication process, offers improved control over mechanical properties, and enhances the resolution of ERISM measurements. The design is compatible with various bio-coatings, maintains long-term cell viability, and significantly improves optical transmission. This enables integrated and confocal fluorescence imaging with improved contrast over traditional ERISM microcavities.