Fluorescent Image Acquisition on VS200 v1

This protocol details the use of the Olympus (Evident Scientific) VS200 automated slide-scanning microscope for fluorescent imaging of fixed mouse, human, and nonhuman-primate tissues sections mounted on barcoded glass 1x3” or 2x3” slides. The instrument is capable of loading up to 210 1x3” glass slides, or 105 2x3” slides. The images are viewable in Evident’s proprietary OlyVIA image viewing software. This document is intended to describe routine use of the instrument and software, including the initial steps required to configure the software for image capture (section 8.3), which includes specific exposure times for the Viral Genetic Tools (VGT) primary screen.

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Publication Details

Published
2025-09-19
DOI
https://doi.org/10.17504/protocols.io.n92ld67rog5b/v1
Primary Topic
Image Processing Techniques and Applications
Type
article
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article

Fluorescent Image Acquisition on VS200 v1

Allen Institute
Image Processing Techniques and Applications
article

Fluorescent Image Acquisition on VS200 v1

Allen Institute
article en

Abstract

This protocol details the use of the Olympus (Evident Scientific) VS200 automated slide-scanning microscope for fluorescent imaging of fixed mouse, human, and nonhuman-primate tissues sections mounted on barcoded glass 1x3” or 2x3” slides. The instrument is capable of loading up to 210 1x3” glass slides, or 105 2x3” slides. The images are viewable in Evident’s proprietary OlyVIA image viewing software. This document is intended to describe routine use of the instrument and software, including the initial steps required to configure the software for image capture (section 8.3), which includes specific exposure times for the Viral Genetic Tools (VGT) primary screen.

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Image Processing Techniques and Applications
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Fluorescent Image Acquisition on VS200 v1 — Allen Institute · (2025) | TGRS Research Map | TGRS