Molecular Analysis and Biochemical Profiling of Bacteria from Domestics Dogs Saliva: A Pilot Study

Microbiota is the collection of microorganisms living in a particular environment, such as the dog’s saliva. The aim of the present work was the detection of bacteriome that plays a crucial role in various ecological processes and has a significant impact on the health and functioning of their host organisms. We collected saliva samples from five dogs. After that, identify the unknown bacterial species by inoculating samples into the Swapped Mannitol Salt Agar (MSA) and MacConkey (MAC). In addition, These bacterial isolates were identified by using morphological characteristics, biochemical tests, and partial sequencing of their 16S rRNA gene.Results] appeared in physical tests on MSA D1, D3, and D4 as staphylococcus; however, in D2, micrococcus appeared. As for MAC, it appeared in D2, D3, D4, and D5 bacillus. The bacterial isolated were identified as Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Staphylococcus pseudintermedius, and Scandinavium goeteborgense. As for antibiotic tests, samples D2-MAC, D3MAC, and D4-MAC have a higher resistance to 83% of antibiotics, such as Penicillin G, Erythromycin, and Ampicillin, while they were susceptible to Cotrimoxazole. On the other hand, D5-MAC was resistant to antibiotic. In Conclusion: This study provides valuable insights into the potential pathogenicity of bacteria isolated from dog saliva. In addition, their antibiotic resistance profiles highlight the need for prudent antibiotic use in human and veterinary medicine.