Comparative Analysis of Plasma Extracellular Vesicle Isolation Methods for Purity Assessment and Biomarker Discovery
SEC provides the most EV-enriched plasma isolates for proteomics information, with minimal contamination from plasma proteins. The PRM-based purity scoring offers an objective means of benchmarking EV preparations and may help standardize EV isolation quality for both biomarker discovery and therapeutic manufacturing.
Authors
- Arsalan S. Haqqani (ORCID: https://orcid.org/0000-0003-2454-8989)
- Melissa Hewitt (ORCID: https://orcid.org/0000-0003-4452-5207)
- Tammy‐Lynn Tremblay
- Alexandra T. Star (ORCID: https://orcid.org/0000-0001-9524-4312)
- William G. Willmore (ORCID: https://orcid.org/0000-0003-3874-7954)
- Jagdeep K. Sandhu (ORCID: https://orcid.org/0000-0002-0065-1335)
- AmanPreet Badhwar (ORCID: https://orcid.org/0000-0003-3414-3395)
- J. Hill (ORCID: https://orcid.org/0000-0003-0031-0706)
- Wen Ding (ORCID: https://orcid.org/0000-0003-1045-7592)
Institutions
- University of Ottawa (CA)
- Institut Universitaire de Gériatrie de Montréal (CA)
- National Research Council Canada (CA)
- Ottawa Institute of Systems Biology
- Carleton University (CA)
Publication Details
- Journal
- Proteomes
- Published
- 2025-09-18
- DOI
- https://doi.org/10.3390/proteomes13030045
- Citations
- 3
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 2.42