Combined GC–MS and RNA-Seq Identification of the Role of the ABC Gene Family in the Formation of Mango Flavor Compounds

<b>Background:</b> Mango is a tropical fruit that is deeply loved by consumers due to its unique flavor and taste. Different mango varieties have unique aromas, and the volatile components of mango are an important part of determining mango flavor. ATP-binding cassette (ABC) transporters are important in transporting plant volatile components. Although ABC transporters have been extensively studied in other species, little is known about the evolutionary characteristics and biological functions of the ABC family in mango. <b>Results:</b> In this study, a total of 119 <i>MiABC</i> genes were identified from the <i>Mangifera indica</i> genome and classified into eight subfamilies based on phylogenetic relationships. By analyzing the gene structure, subcellular localization prediction, chromosome localization, gene duplication events, and <i>Ka</i>/<i>Ks</i> ratios of <i>MiABC</i> genes, the <i>MiABC</i> gene functions were preliminarily determined. The expression profiles of <i>MiABC</i> genes at different stages of mango fruit harvesting indicate that <i>MiABC</i> genes are involved in the transport of volatile substances in mango fruit. The prediction of the transmembrane structure indicates that the <i>MiABC</i> genes have multiple transmembrane domains, and subcellular localization results show that the <i>MiABC</i> genes are mainly located on the cell membrane. <b>Conclusions:</b> In summary, this study conducted a comprehensive analysis of the ABC gene family in mango, laying an important theoretical foundation for the analysis of the transport process of volatile compounds in mango.