An RNA sequencing dataset from a porcine immortalized pre-adipocyte cell line

There is a significant need for livestock cell lines that can be robustly expanded in culture while maintaining their functional characteristics, both for use as <i>in vitro</i> models to understand animal physiology and for industrial applications such as in the emerging sector of cellular agriculture. Here we describe RNA sequencing datasets from a spontaneously immortalized pre-adipocyte line that was derived through serial passaging of porcine adipose-derived mesenchymal stromal cells (MSCs). This cell line, known as FaTTy, is unique in that it displays enhanced adipogenic capacity during long-term culture, characterised by a close to 100% differentiation efficiency and the ability to generate mature adipocytes. Bulk RNA sequencing was performed from FaTTy and parental MSCs. We present analysis of the raw files and bioinformatics analyses, including differential gene expression. These data provide valuable insight on the mechanisms of cell immortalization and the unique phenotype of the FaTTy cell line, with distinctive advantages as a prospective cell source for cultivated fat manufacture.