Addressing Manufacturing and Cost Challenges Toward Solving Low-Cost In Situ Digital Holographic Microscopy Problems

Digital holographic microscopes provide a microscopy solution with a resolution in the low-micrometre range that offers similar performance to optical microscopy, but as a relatively low-cost alternative. The most significant cost saving is due to the ability to reconstruct microscopic images from holograms using low-cost components without the need for an optical stack. The cost saving opens up the avenue towards a feasible solution for geographically distributed in situ microscopic sensing in rural areas for problems like air and water pollution monitoring. The most significant contributors to cost are the camera sensor module, the pinhole, and the processing platform. The latter two components are addressed, at least in part, in this work. We successfully manufactured sub-100 μm diameter pinholes using ultraviolet (UV) laser cutting with an LPKF printed circuit board (PCB) prototyping platform and present the low-cost micromachining method. The pinholes were utilised within a prototype field-programmable gate array (FPGA) demonstrator that successfully reconstructed the holographic images. The choice for the FPGA approach as the initial step, albeit more complex, lends itself towards the easier development of a dedicated reconstructed application-specific integrated circuit (ASIC) to ultimately drive the cost down even further.