Use of prf and ozone therapy for bone regeneration in apical periodontitis defects without the use of grafting material: 3 Case Reports with up to 4-year follow-up

The regeneration of periapical defects remains a significant challenge in dentistry, necessitating innovative therapeutic approaches to restore bone and periodontal integrity. This article explores the combined use of Platelet-Rich Fibrin (PRF) and ozone therapy as an alternative to traditional grafting methods. PRF, derived from the patient's blood, is rich in growth factors like TGF-β, PDGF, EGF, and VEGF, which promote bone regeneration and tissue healing. Ozone therapy, known for its antimicrobial and anti-inflammatory properties, complements PRF by reducing bacterial load and modulating inflammation, further enhancing the regenerative process. This study reports three cases where PRF and ozone therapy were used to treat periapical defects without grafting material, showing promising results. Over a four-year follow-up period, the patients exhibited complete or near-complete healing of bone defects, as evidenced by computed tomography scans. The synergy between PRF and ozone therapy accelerates recovery, reduces treatment times, and improves overall outcomes, making it a viable, less invasive alternative for patients with periapical lesions.