DISORDERS OF LIPID METABOLISM AND ACTIVITY OF ENZYMES OF KREBS CYCLE AND PENTOSE PHOSPHATE PATHWAY IN THE MODELLING OF METABOLIC ACIDOSIS AND ALKALOSIS IN RATS

The aim was to investigate changes in the orientation of cholesterol metabolism and the spectrum of lipids, as well as the activity of key enzymes of the Krebs cycle and the pentose phosphate pathway, regulators of the NADPH/NADP+ ratio, in tissues of rats with disturbances of acid-base state – metabolic acidosis and alkalosis modelled with the aim of unbalanced nutrition: high-protein and carbohydrate (sucrose) diets.Materials and methods. Metabolic acidosis and alkalosis were modelled in rats sticking to diets with excessive ammonium chloride and sucrose. Acid-base balance and lipoprotein cholesterol content were studied in rat blood using conventional methods, then the animals were decapitated, and lipid content and enzyme activity (NADP-dependent IDH and MDH, G6PD and lipase) were determined in liver tissue, cardiac and thigh muscles using TLC.Results and discussion. The development of metabolic acidosis and alkalosis under the influence of diets with excessive ammonium chloride and sucrose was noted. The diet with ammonium chloride promoted lipolysis, activation of lipase, accumulation of under oxidised FFA and cholesterol, decreased the activity of NADP-dependent ICH in all tissues observed and the activity of NADP-dependent MDH in the tissues of the thigh and cardiac muscles of rats, and increased the activity of G6PD in the liver. The sucrose diet increased lipogenesis, cholesterol biosynthesis, and accumulation of FFA in the tissues of the heart and liver, and activated NADP-dependent MDH and IDH in all tissues observed of rats.