A Backwards Approach to GD2 Immunofluorescence in Human Neuroblastoma Tissue Samples: From Staining to Slicing
We were able to establish and validate a novel protocol to reliably perform immunostaining of the membrane antigen GD2 in unfixed, primary neuroblastoma tissue. Although including few limitations, this staining workflow enables relatively quick assessment of GD2 status and thus, might represent a relevant diagnostic tool within the framework of tumor staging and precision medicine.
Authors
- Júlia Florêncio Carvalho Ramos (ORCID: https://orcid.org/0000-0002-0446-8167)
- Laia Pagerols Raluy
- Konrad Reinshagen (ORCID: https://orcid.org/0000-0001-7142-8758)
- Clara Volz
- Isabelle Ariane Bley (ORCID: https://orcid.org/0000-0001-8220-7002)
- Magdalena Trochimiuk
- Sara Peggion (ORCID: https://orcid.org/0000-0002-9703-6434)
Institutions
- Universität Hamburg (DE)
- University Medical Center Hamburg-Eppendorf (DE)
- Kinderkrebs-Zentrum Hamburg (DE)
- Institut Kaplan (ES)
Publication Details
- Journal
- Cells
- Published
- 2025-09-18
- DOI
- https://doi.org/10.3390/cells14181462
- Primary Topic
- Neuroblastoma Research and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00