Discovery of an SQS-PSY Domain-Containing Protein in Meloidogyne incognita Reveals Its Function in Parasitism

Proteins containing the SQS-PSY domain, which include squalene synthetase (SQS), phytoene synthetase (PSY), and NDUFAF6, are functionally important and widely distributed in plants and animals. However, they have not been previously reported in nematodes. In this study, we identified a gene (<i>Minc31999</i>) encoding an SQS-PSY domain-containing protein in the root-knot nematode <i>Meloidogyne incognita</i>. In silico comparison and enzymatic assays of the recombinant protein indicated that this nematode protein is a putative NDUFAF6 homolog. Phylogenetic analysis revealed that this protein is evolutionarily conserved within the Nematoda phylum. RT-qPCR analysis showed that <i>Minc31999</i> is highly expressed during the early infection stage of <i>M. incognita</i>. Targeting the nematode gene <i>Minc31999</i> via host-induced gene silencing (HIGS) significantly hindered nematode development and virulence. In contrast, heterologous expression of <i>Minc31999</i> in <i>Arabidopsis thaliana</i> disrupted normal plant development and increased host susceptibility to nematode infection. Transcriptomic profiling (RNA-seq) of these transgenic plants prior to infections showed a widespread differential expression of genes across multiple metabolic pathways. We propose that this nematode SQS-PSY domain-containing protein may function as an effector that rewires host secondary metabolism to establish a parasitic relationship. Our study elucidates a novel strategy in nematode-plant interactions and advances our understanding of the functional evolution of SQS-PSY domain-containing proteins.