Anticytokine activity of microorganisms isolated in chronic bacterial prostatite

The purpose of the present study was to evaluate the anticytokine activity of opportunistic micro-organisms isolated from the prostate gland secretions in patients with chronic bacterial prostatitis (CBP). In vitro testing was performed with 50 strains of pathogenic bacterial species isolated from the prostate secretions of males who had complaints and confirmed diagnosis of CBP. Isolated bacteria included different Staphylococcus species (Staphylococcus aureus, S. haemolyticus, S. epidermidis), Enterococcus faecalis и Escherichia coli. To assess the anti-cytokine activity (ACA) of bacteria in relation to cytokines of IL-4, IL-6, IL-8, IL-17A and tumor necrosis factor alpha (TNF α), ELISA technique was used using the Cytokine SLR kits (St. Petersburg). The obtained data demonstrated that the anticytokine activity is widespread among various types of conditionally pathogenic bacteria isolated from the prostate gland secretions in patients with CBP. In particular, we have found that the isolates of S. aureus show statistically significant differences in the ACA level towards IL-6 and IL-17A. The cultures of S. haemolyticus were active in relation to IL-4 compared to other studied micro-organisms. Meanwhile, the expression of ACA against IL-8 and TNF α did not differ for different bacterial species. Anticytokine activity may be considered an important marker of bacterial survival under conditions of prolonged interaction with factors of local host immunity in CBP. Further studies in this direction can give new practically useful insights, including those relating to the development of new approaches to the diagnosis of chronic bacterial prostatitis.