Monitoring the epizootic state of apiaries in the Tyumen Region and other regions of Russia

Purpose of the study: continue monitoring apiaries in the Tyumen region and other regions of Russia for contamination of bee colonies with pathogens causing invasions and infections. Research objectives: to study the spread of varroatosis, nosematosis and tropilelapsosis in apiaries of the Tyumen region and other regions of Russia. A total of 181 samples of bees and brood from bee colonies in 21 apiaries, located in the Altai territory, Irkutsk, Tyumen regions and in Dagestan were studied. The studies were conducted in accordance with the “Methodological guidelines for laboratory studies of honey bee nosematosis”, “Methodological guidelines for differential diagnostics of Nosema apis and Nosema ceranae”, “Methodological recommendations for studying means and methods of combating the Varroa mite”, “Methodological guidelines for diagnostics of bee tropilelapsosis”, “Standard methods for Tropilaelaps”. Analysis of the results showed, that the bee colonies have a high level of infection of bees with Varroa. destructor mites (55.5–83.3%), microsporidia N. ceranae (23.1–83.3%), while the degree of infection with nosema ranged from 30.5 to 128 million spores per bee.In the studied pathological material from the Dagestan , Varroa destructor and Tropilaelaps spp. mites were found. The level of infestation of brood cells with the found mites was 55.5%, with Tropilaelaps mites registered in 14.5% of the studied cells. Larval stages of both mites were also found, indicating active reproduction and development of these ectoparasites in bee brood. For the first time in the Tyumen region, Tropilaelaps mercedesae mites, the causative agents of tropilelapsosis in bees, have been found and identified.