Improvement of the process of cryopreservation of cattle sperm with molecular hydrogen

The effect of molecular hydrogen on the functional parameters of bovine sperm cells has been studied. The influence of molecular hydrogen on motility, ATP content, oxidative processes, viability and morphology of the acrosome in bull spermatozoa has been studied. The study was performed on sperm production of black-and-white cattle. The sperm was diluted with a sterile BioXcell medium (France). To analyze the effect of molecular hydrogen on bull spermatozoa, molecular hydrogen was added to the BioXcell medium. The analyzed parameters were studied in native sperm diluted with BioXcell medium, in sperm after deep freezing, as well as sperm subjected to deep freezing and pretreatment with molecular hydrogen. The addition of molecular hydrogen to the medium for diluting sperm contributed to an increase in cell mobility, increased energy metabolism and reduced oxidative stress of spermatozoa. The results obtained showed the need for an in-depth study of the effect of molecular hydrogen on the qualitative characteristics of sperm in cattle and, on this basis, to clarify the existing technological regulations for seed conservation.