Characterization And Molecular Detection of blaIMP Gene from Clinical Isolates of Pseudomonas aeruginosa and Its Implications on Antibiotic Resistance and Biofilm Formation

Background: This research is centered on identifying the blaIMP gene in samples of Pseudomonas aeruginosa providing insights into its impact on resistance and the formation of biofilms by utilizing methods and enhancing persistence and complicating treatment, for infections triggered by this microorganism. Objectives: molecular detection of blaIMP gene, in Pseudomonas aeruginosa samples obtained from patients and evaluation of its relationship to resistance against antibiotics and the development of biofilms. Comprehend the significance of the blaIMP gene in terms of resistance and its impact on biofilm-related persistence. Investigate approaches that can be used to tackle resistance caused by blaIMP and the formation of biofilms, in case of Pseudomonas aeruginosa infections. Materials and methods: Twenty Pseudomonas aeruginosa isolates were collected from Al-Nahrain University's College of Biotechnology to test the development of biofilms, identify virulence genes using molecular means, and discover ways to reduce antibiotic resistance. Results: The results of biochemical detection of 20 Pseudomonas aeruginosa isolates revealed that bacterial isolates were negative for indole while Citrate and catalase were positive and these isolates were pyocyanin-producing and sensitive to imipenem, Doxycycline, Levofloxacin, Cefotaxime, gentamicin, and aztreonam with an inhibition zone of 10,6,2,0,10,8mm respectively. The results of biofilm formation of selected 20 isolates indicated that 10% weak, 30% moderate,60% strong, and non-biofilm producer. Also, PCR amplification used to detect bacterial isolates as Pseudomonas aeruginosa revealed that bands appeared in amplification by 16S rRNA gene and the bla IMP gene. Results of the antibacterial activity of quantum dots nanoparticles on Pseudomonas aeruginosa showed that at four concentrations tested (100,75,50 and 25 μg/ml), all tested isolates showed an inhibition zone. Finally, the results of cytotoxicity assays of nanoparticles using MTT assay on the HepG2 liver cancer line revealed.