New compounds with anti-inflammatory activity from the roots of <i>Isatis indigotica</i>

Four new compounds, isatigotindoledioside G (<b>1</b>), methyl-9<i>S</i>,12<i>S</i>,13<i>S</i>-trihydroxyoctadeca-10<i>E</i>,15<i>E</i>-dienoate (<b>2</b>), 9<i>S</i>,12<i>S</i>,13<i>S</i>-trihydroxyoctadeca-10<i>E</i>,15<i>E</i>-dienoic acid (<b>3</b>), and one new artificial product ethyl 9<i>S</i>,12<i>S</i>,13<i>S</i>-trihydroxyoctadeca-10<i>E</i>,15<i>Z</i>-dienoate (<b>4</b>), along with fourteen recognized compounds (<b>5-18</b>), were extracted from the roots of <i>Isatis indigotica</i>. These compounds' structures were characterized using NMR spectroscopy, HR-ESI-MS, ECD calculations, and literature comparisons. Compounds <b>5-6</b> and <b>10-11</b> showed appreciable anti-inflammatory properties, and compounds <b>1-4</b>, <b>12-13</b>, and <b>17-18</b> showed mild anti-inflammatory properties when evaluated in the LPS-stimulated RAW264.7 cell model.