miR-30c-5p serves a diagnostic biomarker in chronic heart failure patients and its regulatory role in vascular endothelial

Abstract Objectives Chronic heart failure (CHF) is a syndrome of conflicting organ supply and demand caused by cardiac insufficiency. Previous studies have demonstrated that miR-30c-5p is strongly associated with cardiovascular diseases such as cardiac hypertrophy and acute coronary syndrome. The aim of this study was to explore the meaning of miR-30c-5p in the diagnosis of CHF patients and its effect on vascular endothelial cells. Methods Serum miR-30c-5p levels were detected in 113 CHF patients and 98 healthy individuals by RT-qPCR. ROC curve and logistic regression analysis were conducted to estimate the diagnostic ability and risk factors of miR-30c-5p for CHF. The relationship of miR-30c-5p with BNP and LVEF was analyzed by Pearson correlation. The influence of miR-30c-5p on HUVECs cell lines was explored by transfection with miR-30c-5p mimic/inhibitor. Cell apoptosis and proliferation were detected by flow cytometry and CCK8 assay. Transwell assay was used to detect the migration and invasion abilities of cells. Results miR-30c-5p was reduced in CHF patients’ serum and progressively decreased with increasing NYHA grade. The ROC curve demonstrated that miR-30c-5p had high sensitivity (83.19 %) and specificity (84.69 %) to diagnose CHF. Logistic regression revealed that miR-30c-5p was a pivotal risk factor for CHF development. Pearson correlation analysis suggested that miR-30c-5p was negatively related to BNP and positively correlated with LVEF. Transfection of miR-30c-5p mimic suppressed apoptosis and promoted cell proliferation, migration, and invasion in HUVECs. Conclusions Reduced serum miR-30c-5p levels in CHF patients may be a biomarker for CHF diagnosis. Aberrant miR-30c-5p expression may influence CHF development by affecting the HUVECs function.