Integrated biological and chemical investigation of papuan marine organisms for the discovery of potential cytotoxic marine natural products
An integrated approach combining biological and chemical investigations were carried out to uncover novel cytotoxic molecules from diverse Papuan marine organisms. Of 386 marine-derived extracts, about 13% (50 extracts) showed significant toxicity in the <i>Artemia salina</i> lethality assay (LC<sub>50</sub> ≤ 5 µg/mL). Analysis of the Z-factor and Z'-factor confirmed the excellent quality of the assay (<i>Z</i> = 0.76, Z' = 0.5). Of the 50 active extracts, seven extracts from <i>Achantostrongylophora ingens</i>, <i>Theonella</i> sp., <i>Ianthella basta</i>, a cyanobacterium, a sponge from family Thorectidae, and a rare <i>Axinella</i> sp. were further evaluated for cytotoxicity against HEK293T, <sup>1</sup>H NMR spectral, LC-HR-MS/MS, feature-based molecular networking (FBMN), and chemoinformatics analyses. The chemical analyses revealed the presence of 43 known and 381 putatively new bioactive molecules. The integrated biological and chemistry approach adopted in this study is effective for prioritising important marine extracts with high probability of discovering novel cytotoxic compounds.
Authors
- Fitriya N. Dewi (ORCID: https://orcid.org/0000-0002-7908-7879)
- Emmanuel T. Oluwabusola (ORCID: https://orcid.org/0000-0003-3153-4323)
- Junichi Tanaka (ORCID: https://orcid.org/0000-0002-8672-7379)
- Siti Sa’diah (ORCID: https://orcid.org/0000-0001-7513-4431)
- Trianda Ayuning Tyas (ORCID: https://orcid.org/0009-0001-2220-2004)
- Marcel Jaspars (ORCID: https://orcid.org/0000-0002-2426-6028)
- Takahiro Jomori (ORCID: https://orcid.org/0000-0001-5866-0341)
- Lik Tong Tan (ORCID: https://orcid.org/0000-0003-0138-4539)
- Yuta Tsunematsu (ORCID: https://orcid.org/0000-0001-6750-8034)
- Kusdiantoro Mohamad (ORCID: https://orcid.org/0000-0002-7143-168X)
- Anggia Murni (ORCID: https://orcid.org/0000-0001-7557-7849)
- Novriyandi Hanif (ORCID: https://orcid.org/0000-0001-9570-3750)
- Masaki Kita (ORCID: https://orcid.org/0000-0001-6685-4461)
- Nicole J. de Voogd (ORCID: https://orcid.org/0000-0002-7985-5604)
- Ekowati Chasanah
- Jonathan Stefanson Pakan
- Shannon Christela Lisias
- Wildan Bachtiar Sael
- Nurul Fajar Santosa
- Martinus Mario Lemuel
Institutions
- Leiden University (NL)
- Naturalis Biodiversity Center (NL)
- Nanyang Technological University (SG)
- University of Aberdeen (GB)
- IPB University (ID)
- Discovery Centre (CA)
- University of the Ryukyus (JP)
- National Agricultural Research and Innovation Centre (HU)
- Indonesian Agency for Agricultural Research and Development (ID)
- National Research and Innovation Agency (ID)
- Nagoya University (JP)
Publication Details
- Journal
- Natural Product Research
- Published
- 2025-09-16
- DOI
- https://doi.org/10.1080/14786419.2025.2559778
- Primary Topic
- Marine Sponges and Natural Products
- Type
- article
- Field-Weighted Citation Impact
- 0.00