A user‐friendly web tool for colloidal interaction analysis: leveraging the <scp>xDLVO</scp> theory in integrated bioprocessing

Abstract BACKGROUND The extended Derjaguin, Landau, Verwey, and Overbeek (xDLVO) theory is a well‐known framework for the evaluation of the forces that determine colloidal stability and aggregation in various scientific domains, including integrated biomanufacturing. To apply this theory to the understanding and prediction of colloidal interactions, complex calculations must be implemented. We developed a web application to provide researchers and practitioners with a tool that streamlines the application of xDLVO theory. RESULTS The simple interface allows users to insert experimental data and parameters in addition to generating graphs and tables which provides insight into the forces governing biomaterial interactions. This web tool also facilitates the exploration of pre‐existing data from a database and the input of new experimental values, promoting flexibility and adaptability. We applied it in the analysis of biomass‐matrix interactions within several direct adsorption systems, including a range of commercially available adsorbents and biomass types. The calculated parameters replicate the interaction energies which determine the force of these interactions, providing a predictive tool for optimizing experimental conditions in process biotechnology. CONCLUSION This web application provides an effective and user‐friendly platform for the investigation of colloidal interactions, making it a valuable resource for the scientific community. Its potential application on a variety of scientific fields is demonstrated by its transdisciplinary applications. We want to make sure that this web tool meets the needs of researchers investigating colloid and surface science, contributing to further bioprocess development. © 2025 Society of Chemical Industry (SCI).