Photo‐Responsive Nano‐Enzyme Catalysis for Controlled ROS Generation

Abstract The ability to modulate Reactive Oxygen Species (ROS) generation based on light exposure could be applied in various clinical settings, such as wound healing, cancer therapy, and stem cell maintenance, where precise ROS regulation is crucial. This study presents the light‐controlled ROS generation properties of ZIF‐8@BQ nanohybrid, which were explored, showing its potential as a smart, light‐regulated ROS generator, offering a novel approach for balancing ROS levels in therapeutic applications. The ZIF‐8@BQ nanohybrid exhibited a significantly enhanced photoinduced ROS production, which was predominantly oxygen radicals, as confirmed by a DCFH assay. Additionally, the ZIF‐8@BQ nanohybrid represents a promising nanomaterial for advanced therapeutic strategies, with the potential for tailored ROS‐based treatments in a wide range of biomedical applications. The UV–vis and fluorescence spectra, along with various analytical techniques such as SEM, TEM, and zeta potential measurements, demonstrated successful hybridization of Benzoquinone (BQ) with ZIF‐8 nanoparticles (NPs), indicating alterations in the electronic properties of the hybrid material. The SEM and TEM results confirmed the formation of uniform rhombic dodecahedron ZIF‐8 NPs with dimensions around 170–200 nm, with the incorporation of BQ resulting in minor structural changes.