Nitrogen-fixing Ability of a Thermophilic Sulfate-reducing Bacterium in the Genus <i>Thermodesulfovibrio</i> Isolated from a Terrestrial Hot Spring in Japan
Nitrogen-fixing sulfate-reducing bacteria have not yet been exami-ned in thermal environments. In this study, strain TK110, belonging to the genus Thermodesulfovibrio, was successfully isolated from a geothermal spring using an NH<sub>3</sub>-free inorganic medium. Strain TK110 harbored genes associated with the Calvin-Benson-Bassham cycle and nitrogen fixation-related genes, nifHDKENXIIB. Nitrogenase activity was assessed using an acetylene reduction assay and detected in strain TK110 under autotrophic conditions, as well as in Thermodesulfovibrio yellowstonii DSM 11347<sup>T</sup> under heterotrophic conditions at 65°C. To the best of our knowledge, this is the first study to demonstrate nitrogen fixation by thermophilic sulfate-reducing bacteria.
Authors
- Arisa Nishihara (ORCID: https://orcid.org/0000-0003-0907-4818)
- Shin Haruta (ORCID: https://orcid.org/0000-0002-3705-2459)
- Toko Hisano
Institutions
- Tokyo Metropolitan University (JP)
- National Institute of Advanced Industrial Science and Technology (JP)
Publication Details
- Journal
- Microbes and Environments
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1264/jsme2.me25030
- Citations
- 1
- Primary Topic
- Microbial Community Ecology and Physiology
- Type
- article
- Field-Weighted Citation Impact
- 1.72
Funders
- Japan Society for the Promotion of Science