Development And Validation Of UV Spectrophotometric Methods For Quantification Of Edaravone And Application Of Greenness And Blueness Study

This work presents a simple, rapid, accurate and precise UV-spectrophotometric methods for estimation of Edaravone in bulk and its pharmaceutical preparation using an Acetonitrile and Water 5:5 mixture as solvent. Method A was performed using maximum absorbance zero order derivative method with 242nm and Method B was based on Zero order Area under Curve method with wavelength from 230nm to 244nm. The development of the method was validated as per ICH Q2 (R1) guidelines. The methods showed good linear response across a concentration range 2-12μg/ml with r2 value of 0.999 respectively. The percentage Relative Standard Deviation was found to be less than 2, indicating the method was precise. Additionally, the methods greenness were assessed by means of ecological tools which were up-to-date, namely, AGREE (Analytical Greenness Metric Approach), MoGAPI (Modified Greenness Analytical Procedure Index) for greenness. The methods were also assessed for blueness using BAGI (Blue Applicability Grade Index) a recently developed metric for assessing practicality of procedure.