Revisiting biosensing using digital holography

Multiplexed analyte sensing in biological samples is crucial for detecting diseases and therapeutic monitoring, yet remains a challenge because of sample heterogeneity. This talk introduces a novel approach combining digital holography, microfluidics, and engineered supported lipid bilayers for label-free biosensing. We demonstrate size-specific, single particle-based, multiplexed immunoassays targeting extracellular vesicles and proteins. Beyond fingerprinting heterogeneous populations, by leveraging polarization-sensitive holographic imaging, we also reveal spatially resolved chirality, including enantiomeric excess, normally obscured by ensemble averaging. Our approach unlocks new dimensions in biosensing, paving the way for advanced diagnostics and fundamental biological insights.