An Ultra-Sensitive Aflatoxin B<sub>1</sub> Electrochemical Immunosensor Based on a Site-Directed Antibody Conjugation Strategy

Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) is one of the most carcinogenic and highly toxic mycotoxins, posing significant risks to human and animal health. Accurate and sensitive detection of AFB<sub>1</sub> is essential for ensuring food safety. In this study, we designed an ultrasensitive electrochemical immunosensor based on an AuNP-TDN-SPA-mAb immunoprobe for AFB<sub>1</sub> detection. This immunoprobe was developed using a site-directed antibody conjugation strategy, which enables dual modulation of antibody orientation and programmable surface distribution, thereby enhancing detection performance. Experimental results showed that the developed immunosensor exhibited a linear response range from 0.05 to 5000 pg/mL, with a limit of detection as low as 0.042 pg/mL. The immunosensor was successfully applied to the analysis of wheat and rice bran oil samples, yielding results consistent with those obtained using ultraperformance liquid chromatography (UPLC). Therefore, this site-directed antibody conjugation approach shows significant potential for broad application in the sensitive detection of mycotoxins to guarantee food safety.

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Publication Details

Journal
Journal of Agricultural and Food Chemistry
Published
2025-09-15
DOI
https://doi.org/10.1021/acs.jafc.5c09690
Primary Topic
Advanced biosensing and bioanalysis techniques
Type
article
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article

An Ultra-Sensitive Aflatoxin B1 Electrochemical Immunosensor Based on a Site-Directed Antibody Conjugation Strategy

Zhiyong Gong, Min Fang, Qing Yang, Lin Xu et al.
Journal of Agricultural and Food Chemistry
Advanced biosensing and bioanalysis techniques
article

An Ultra-Sensitive Aflatoxin B1 Electrochemical Immunosensor Based on a Site-Directed Antibody Conjugation Strategy

Zhiyong Gong, Min Fang, Qing Yang, Lin Xu, Xin Liu, Xiaotong Hao, Huang Dai, Zhengwei Zhu
article en

Abstract

Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) is one of the most carcinogenic and highly toxic mycotoxins, posing significant risks to human and animal health. Accurate and sensitive detection of AFB<sub>1</sub> is essential for ensuring food safety. In this study, we designed an ultrasensitive electrochemical immunosensor based on an AuNP-TDN-SPA-mAb immunoprobe for AFB<sub>1</sub> detection. This immunoprobe was developed using a site-directed antibody conjugation strategy, which enables dual modulation of antibody orientation and programmable surface distribution, thereby enhancing detection performance. Experimental results showed that the developed immunosensor exhibited a linear response range from 0.05 to 5000 pg/mL, with a limit of detection as low as 0.042 pg/mL. The immunosensor was successfully applied to the analysis of wheat and rice bran oil samples, yielding results consistent with those obtained using ultraperformance liquid chromatography (UPLC). Therefore, this site-directed antibody conjugation approach shows significant potential for broad application in the sensitive detection of mycotoxins to guarantee food safety.

Journal of Agricultural and Food ChemistryVol. 73(38)
Wuhan Polytechnic University (CN), National Engineering Research Center for Wheat (CN), Institute of Food Science & Technology (GB), Shandong Special Equipment Inspection Institute (CN)
Openalex Percentile: Top 78%
Advanced biosensing and bioanalysis techniques
DOISourceOpenAlex
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An Ultra-Sensitive Aflatoxin B<sub>1</sub> Electrochemical Immunosensor Based on a Site-Directed Antibody Conjugation Strategy — Zhiyong Gong, Min Fang, et al. · Journal of Agricultural and Food Chemistry (2025) | TGRS Research Map | TGRS