The immunomodulatory and antitumor properties of the bacterial metalloprotease Oligopeptidase A are mediated by TLR4/MyD88/TRIF and MAPK signaling pathways
Heat-inactivated OpdA significantly reduced metastasis, dependent on tumor-specific CD4+ and CD8+ T cells and IFN-γ, both locally and systemically, with decreased IL-10 levels suggesting a proinflammatory environment. Treatment increased secretion of nitric oxide, IL-12p40, and TNF-α from bone marrow cells via enzymatic activity, involving MyD88/TRIF and MAPK pathways. Conclusion: OpdA shows potential as a tumor vaccine adjuvant, promoting antigen presentation and tumor-specific immune responses.
Authors
- Priscila P. Silva (ORCID: https://orcid.org/0000-0002-9983-3840)
- Samanta Lopes Tomaz
- Rodrigo Berzaghi
- Felipe V. Pereira
- Fellipe Bronze (ORCID: https://orcid.org/0000-0002-5419-9655)
- Tarciso Almeida Sellani
- Carolina de Amat Herbozo (ORCID: https://orcid.org/0000-0001-9216-6590)
- Leopoldo Rugieri Carvalho Vaz da Silva
- Filipe Menegatti de Melo (ORCID: https://orcid.org/0000-0002-3672-3636)
- Isaías Glezer (ORCID: https://orcid.org/0000-0002-9799-1933)
- Thaysa Paschoalin (ORCID: https://orcid.org/0000-0003-4664-2001)
- Marcelo F. Marcondes (ORCID: https://orcid.org/0000-0003-4213-5467)
- Elaine G. Rodrigues (ORCID: https://orcid.org/0000-0002-3248-3081)
- Adriana K. Carmona (ORCID: https://orcid.org/0000-0003-3311-5456)
- Gabrielli Novaes Silva
- Amanda Campelo Lima de Melo
Institutions
- Goethe University Frankfurt (DE)
- University of California, Los Angeles (US)
- Hospital Sírio-Libanês (BR)
- GlaxoSmithKline (Brazil) (BR)
- Hospital São Paulo (BR)
- University of Colorado Anschutz Medical Campus (US)
- UiT The Arctic University of Norway (NO)
- Universidade Federal de São Paulo (BR)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-09-12
- DOI
- https://doi.org/10.3389/fimmu.2025.1630886
- Primary Topic
- Peptidase Inhibition and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00