Cover Feature: Engineering Nicotinamide Adenine Dinucleotide Oxidase for Regeneration of Oxidized Non‐natural Cofactor (ChemBioChem 16/2025)

Oxidative and reductive reactions are vital processes in cellular metabolism. Like yin and yang in Tai Chi, they are independent and complementary. The non-natural cofactors NCD+ and NCDH exhibit substantial potential for the bio-orthogonal regulation of redox pathways. To provide sufficient driving force, it is crucial to maintain the NCD+ and NCDH ratio and homeostasis. In the Research Article 10.1002/cbic.202500254, Xueying Wang, Zongbao K. Zhao, and co-workers explain how they reshaped the NADH-binding pocket of NADH oxidase to accommodate NCDH, thereby facilitating the traceless regeneration of the oxidized cofactor NCD+ and the selective synthesis of chiral compounds.