Impact of a 24-Week Workplace Physical Activity Program on Oxidative Stress Markers, Metabolic Health, and Physical Fitness: A Pilot Study in a Real-World Academic Setting

<b>Background</b>: Previous studies showed that workplace physical activity programs (WPAPs) could improve general health among employees. However, there is a lack of correlation between oxidative redox status and the metabolic and physical fitness (PF) of workers. The objective of the study was to evaluate the improvements of a 24-week combined circuit training and mobility training program on PF, oxidative redox status, and metabolic parameters on healthy academic employees. <b>Methods</b>: Twenty-six university employees (52.8 ± 11.5 years) followed a 24-week WPAP composed of two circuit training sessions and one mobility training session per week. PF components were assessed through one leg stand, shoulder/neck mobility, handgrip, dynamic sit-up, jump and reach, and 2-Minute step test (2MST). Oxidative stress and antioxidant potential were evaluated through derived-Reactive Oxygen Metabolites (d-ROM) and biological antioxidant potential (BAP) tests, respectively. Metabolic measurements included total cholesterol, LDL-C, HDL-C, triglycerides, and fasting plasma glucose. All assessments were conducted at baseline and after 24 weeks. <b>Results</b>: D-ROM values increased significantly likely due to an acute adaptive response to exercise and a stable BAP/d-ROM ratio was maintained. At baseline, subjects with higher 2MST scores showed a better BAP/d-ROM ratio compared to those with lower 2MST scores, which was also associated with normal weight status (<i>p</i> < 0.05), healthy values of triglycerides (<i>p</i> < 0.01), and LDL-C (<i>p</i> < 0.01). Excluding statin-treated subjects, an intriguing shift toward a condition of enhanced antioxidant capacity was observed. <b>Conclusions</b>: Overall, the 24-week WPAP improved metabolic health and maintained redox balance, despite increased reactive oxygen species (ROS) production. Statin supplementation may have hidden antioxidant adaptations to physical exercise, an intriguing observation that warrants further studies.