Biogenic synthesis of Silver nanoparticles using Secondary metabolites of Lactiplantibacillus plantarum and its potential applications

This study investigated the eco-friendly synthesis of silver nanoparticles (AgNP’s) using secondary metabolites derived from probiotic bacteria naturally existing in the human gut, such as Lactiplantibacillus plantarum. Probiotics are well-known for producing a wide range of bioactive compounds, including peptides, organic acids, bacteriocins, and exopolysaccharides, which can act as reducing and capping agents in bio-nanoparticle synthesis. These metabolites serve dual functions: facilitating nanoparticle formation and making their biological functionality. The synthesized AgNP’s were characterized using advanced techniques to determine their size, morphology, and surface chemistry. The study emphasizes their potent antimicrobial, antifungal, and anticancer properties against MCF-7 breast cancer cell lines. Antibacterial activity against Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae. Antifungal efficacy against Aspergillus niger and Aspergillus flavus, demonstrating promising applications in pharmaceuticals and healthcare. Additionally, the nanoparticles were tested for their ability to enhance textile materials by testing their antibacterial activity and durability, thus supporting their role in functional fabrics used in medical clothing, sportswear, and hygienic textiles, which was compared to the chemically synthesized nanoparticles to achieve higher biocompatibility, stability, and sustainability. This work highlights the untapped potential of probiotic microorganisms as a novel and renewable source for nanomaterial production and paves the way for interdisciplinary applications spanning medicine, agriculture, environmental remediation, and smart textiles. Keywords: Lactiplantibacillus plantarum, Silver Nanoparticles, Antibacterial, Antifungal, Anticancer activity