In Vitro Wound Healing and Antibiofilm Potential of β-Glucan and L-EPS Obtained from Lactoplantibacillus plantarum GD2

Purpose: This study aims to evaluate the in vitro wound healing and antibiofilm potential of commercial β-glucan and L-EPS derived from Lactoplantibacillus plantarum GD2. Biocompatibility, cell migration and biofilm formation inhibitory effects of biopolymers were investigated. Materials and Methods: Cytotoxicity was analyzed using the MTT assay on L929 mouse fibroblast and RAW264.7 mouse macrophage cell lines. Antibiofilm activity was assessed based on microbial colonization prevention, while wound healing potential was evaluated using the scratch test. The statistical significance of the results was tested. Results: Both β-glucan and L-EPS were found to be biocompatible in individual and combined applications. Antibiofilm analyses revealed that these biopolymers can prevent microbial colonization, maintaining a sterile wound environment. Scratch test results demonstrated that they promoted cell migration and accelerated wound closure. The combination showed similar or enhanced effects compared to individual applications, suggesting synergistic properties. Conclusion: β-glucan and L-EPS derived from L. plantarum GD2 are promising biomaterials with wound healing acceleration and biofilm prevention properties. However, further in vivo and clinical studies are required to confirm their biomedical potential.