Assessing the Impact of SARS-CoV-2 Spike Mutations on Antibody Binding: A Comparative Assessment of the Wuhan and JN.1 Variants’ Full-Length Spikes in a Multiplex Luminex Assay
Both ELISA and Luminex assays showed comparable sensitivity and specificity for both Wuhan and JN.1 antigens, indicating that mutations in the JN.1 variant do not significantly impact assay performance. This suggests preserved antigenic recognition across variants.
Authors
- Ruth Nyakundi (ORCID: https://orcid.org/0000-0003-4428-5207)
- Charles Sande (ORCID: https://orcid.org/0000-0002-8364-6063)
- J. Anthony G. Scott (ORCID: https://orcid.org/0000-0001-7533-5006)
- Faiz Shee (ORCID: https://orcid.org/0000-0002-1114-6449)
- E. Wangeci Kagucia (ORCID: https://orcid.org/0000-0002-4481-8889)
- Paul Ogongo (ORCID: https://orcid.org/0000-0002-0093-5768)
- Charles N. Agoti (ORCID: https://orcid.org/0000-0002-2160-567X)
- Lucy Ochola (ORCID: https://orcid.org/0000-0003-0264-5526)
- Jennifer Musyoki (ORCID: https://orcid.org/0000-0001-7002-9384)
- Lynette Isabella Ochola‐Oyier (ORCID: https://orcid.org/0000-0003-4393-0470)
- Christina Mwachari (ORCID: https://orcid.org/0000-0002-8650-4079)
- Bernadette Kutima
- John N. Gitonga (ORCID: https://orcid.org/0000-0001-6482-9107)
- Sophie Uyoga (ORCID: https://orcid.org/0000-0003-1746-7873)
- Christopher K. Kariuki (ORCID: https://orcid.org/0000-0001-6596-9304)
- James Nyagwange (ORCID: https://orcid.org/0000-0001-5430-6011)
- George M. Warimwe (ORCID: https://orcid.org/0000-0002-4911-6333)
- Ambrose Agweyu (ORCID: https://orcid.org/0000-0001-8760-1279)
- Martin Omondi Alfred (ORCID: https://orcid.org/0000-0002-5954-311X)
- Doreen Lugano (ORCID: https://orcid.org/0009-0000-9542-0558)
- Sharon Owuor
- Angela Maina (ORCID: https://orcid.org/0009-0004-7844-5744)
- Philip Bejon
- Gloria Konyino
- Gerald Waweru
Institutions
- Pwani University (KE)
- Kenya Medical Research Institute (KE)
- University of Oxford (GB)
- London School of Hygiene & Tropical Medicine (GB)
- Institute of Primate Research (KE)
Publication Details
- Journal
- Viruses
- Published
- 2025-09-16
- DOI
- https://doi.org/10.3390/v17091248
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00