Study on Vasicinone Production and Precursor Feeding from Callus Cultures of Justicia Adhatoda

The establishment of tissue culture methods for several medicinal plants made it possible to analyze callus/cell suspension extractions for the presence of several secondary products. Callus cultures were created in this work using Justicia adhatoda leaves and petioles. Earlier known as Adhatoda zeylanica Medicus on Murashige and Skoog (MS 1962) and Gamborg’s (B5 1976) medium. The extracts of the callus samples were compared with that of authentic Vasicinone by Co-TLC, and analyzed by 1HNMR, 13CNMR and Electron Ionization Mass Spectroscopy. Quantification of the vasicinone in different extracts of callus samples were done by HPLC analysis and their retention time in leaf-derived callus extract is 6.263 and for that of petiole-derived callus extract is 6.357 and both the samples are compared with that of Authentic vasicinone sample (6.2min retention time). Further the productivity of cell lines producing vasicinone was determined by adding different carbon sources viz., Glucose, Fructose, Sucrose, Mannose and aromatic amino acids Phenylalanine, Tyrosine, Ornithine as precursors to the culture medium and tested.