Profiling bile acids in the stools of humans and animal models of cystic fibrosis
Changes in the abundance and/or composition of intestinal BAs may contribute to dysbiosis and altered gastrointestinal physiology in CF. Here, we report shifts in select fecal BA classes and species for cwCF. Matched metagenomic analysis suggests possible defects in both host intestinal BA absorption and gut microbial BA metabolism. Additional analyses of mouse and ferret CF stool for BA composition suggest great care must be taken when interpreting BA functional studies using these animal models. Together, this work lays technical and conceptual foundations for interrogating BA-microbe interactions in cwCF.
Authors
- Rebecca A. Valls
- George A. O’Toole (ORCID: https://orcid.org/0000-0002-2861-4392)
- Sarvesh Surve (ORCID: https://orcid.org/0000-0003-2942-6315)
- Julie Sanville (ORCID: https://orcid.org/0000-0002-4805-5276)
- Mark S. Sundrud (ORCID: https://orcid.org/0000-0002-4249-0525)
- Shannon M. Soucy (ORCID: https://orcid.org/0000-0001-8672-316X)
- Juliette C. Madan (ORCID: https://orcid.org/0000-0003-0542-1368)
- Melissa M. Carmichael
Institutions
- Dartmouth College (US)
- Dartmouth Institute for Health Policy and Clinical Practice (US)
- Dartmouth Psychiatric Research Center (US)
- Hochschule Geisenheim University (DE)
- Dartmouth Hospital (GB)
- Dartmouth Health (US)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-09-16
- DOI
- https://doi.org/10.1128/spectrum.01451-25
- Citations
- 2
- Primary Topic
- Cystic Fibrosis Research Advances
- Type
- article
- Field-Weighted Citation Impact
- 3.04