Dynamic Observation of Neuroprotective Effect of rTMS on BBB in an Ischemic Stroke Rat Model using DCE-MRI combined with Immunofluorescence

Motivation: Repetitive transcranial magnetic stimulation (rTMS) has been widely used in clinical rehabilitation for ischemic stroke. However, there was limited research on the impact of rTMS on blood-brain barrier (BBB). Goal(s): This study investigated the effects of rTMS on BBB in ischemic stroke rats. Approach: Eighty rats were divided into two groups: the rTMS group received 10Hz rTMS for 2 weeks, while the control group received no treatment. Neurological assessments, MRI scanning and immunofluorescence staining were performed at multiple time points: baseline, day 0.25, 3, 7 and 14. Results: 2-week rTMS treatment reduced rKtrans of infarct lesions and infarct volume, while improving neurological outcomes. Impact: This study demonstrates that rTMS can effectively reduce BBB permeability and infarct volume in ischemic stroke, suggesting a potential therapeutic approach to improve neurological outcomes by stabilizing the BBB.