7T 3D-MR Spectroscopic Imaging of GSH and GPX4 Immunohistochemistry Staining Reveal Oxidative Stress Patterns in MS Lesions
Motivation: GSH in MS can reveal oxidative stress areas where the body mounts defence, but the mechanism of GSH counteraction remains poorly understood due to limitations of standard MRS. Goal(s): By mapping GSH distribution across lesion types, we aim to elucidate antioxidative responses in the MS brain, informing potential therapeutic targets. Approach: We used 7T high-resolution echo-less 3D-MRSI to map GSH levels in 22 MS patients and validated findings with GPX4 immunohistochemistry post mortem. Results: The GSH/tCr ratio was elevated in lesions, particularly paramagnetic rim lesions, compared to normal-appearing WM; GPX4 staining indicated higher antioxidative activity in lesions than in normal-appearing and healthy WM. Impact: High-resolution 7T 3D-MRSI mapping of GSH reveals oxidative stress patterns across MS lesions, enhancing in vivo lesion monitoring and offering comprehensive insights that support clinical management and progression tracking in multiple sclerosis.
Authors
- Eva Niess (ORCID: https://orcid.org/0000-0001-9956-1470)
- Günther Grabner (ORCID: https://orcid.org/0000-0001-8330-8446)
- Wolfgang Bogner (ORCID: https://orcid.org/0000-0002-0130-3463)
- Lukas Hingerl (ORCID: https://orcid.org/0000-0003-1808-8349)
- Paulus Stefan Rommer (ORCID: https://orcid.org/0000-0001-5209-6647)
- Simon Hametner (ORCID: https://orcid.org/0000-0002-5160-5208)
- Fabian Niess (ORCID: https://orcid.org/0000-0003-1235-7595)
- Bernhard Strasser (ORCID: https://orcid.org/0000-0001-9542-3855)
- Romana Höftberger (ORCID: https://orcid.org/0000-0002-5769-1100)
- Thomas Berger (ORCID: https://orcid.org/0000-0002-2062-5193)
- Assunta Dal‐Bianco (ORCID: https://orcid.org/0000-0002-4284-3335)
- Anita Kloss‐Brandstätter (ORCID: https://orcid.org/0000-0002-0873-6704)
- Rebeka Rumbak (ORCID: https://orcid.org/0000-0003-1881-5323)
Publication Details
- Journal
- Proceedings on CD-ROM - International Society for Magnetic Resonance in Medicine. Scientific Meeting and Exhibition/Proceedings of the International Society for Magnetic Resonance in Medicine, Scientific Meeting and Exhibition
- Published
- 2025-09-16
- DOI
- https://doi.org/10.58530/2025/3763
- Primary Topic
- RNA regulation and disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00