Diffusion MRI acquisition methods for post-mortem imaging on a 10.5 T whole-body human MRI scanner
Motivation: High-quality post-mortem diffusion MRI + microscopy datasets acquired in the same brain enable novel, cross-scale neuroanatomical investigations. Goal(s): Identify a sequence that maximises SNR-efficiency and diffusion contrast in a post-mortem macaque brain using a 10.5 T whole-body human MRI scanner. Approach: Theoretical SNR-efficiency comparisons were performed between the diffusion-weighted spin-echo (DW-SE), stimulated-echo (DW-STE) and steady-state free precession (DW-SSFP) sequence. We performed a pilot investigation using the leading method in a post-mortem macaque brain. Results: DW-SSFP predicted the highest SNR-efficiency across the target b-value range. Acquired DW-SSFP data (0.4 mm isotropic) demonstrated high-SNR diffusivity estimates without geometric distortions, resolving multiple-fibre populations and long-range tracts. Impact: This research investigates acquisition methods to obtain high-quality post-mortem diffusion MRI datasets using a 10.5 T whole-body human MRI scanner. Data acquired using the described methodology provide detailed neuroanatomical information, informing future brain connectivity and in vivo investigations.
Authors
- Gregor Adriany (ORCID: https://orcid.org/0000-0002-6428-9005)
- Jan Zimmermann (ORCID: https://orcid.org/0000-0003-3345-6074)
- Noam Harel (ORCID: https://orcid.org/0000-0002-6034-3801)
- Matt Waks (ORCID: https://orcid.org/0000-0003-1743-8888)
- Mohamed Kotb Selim (ORCID: https://orcid.org/0000-0001-8610-3147)
- Hamza Farooq (ORCID: https://orcid.org/0000-0001-5311-4368)
- Pramod Kumar Pisharady (ORCID: https://orcid.org/0000-0002-0756-1199)
- Essa Yacoub (ORCID: https://orcid.org/0009-0000-1007-9056)
- Saâd Jbabdi (ORCID: https://orcid.org/0000-0003-3234-5639)
- Shaun Warrington (ORCID: https://orcid.org/0000-0002-7198-8162)
- Stamatios N. Sotiropoulos (ORCID: https://orcid.org/0000-0003-4735-5776)
- Alexander Bratch
- Edward J. Auerbach (ORCID: https://orcid.org/0000-0003-4553-1545)
- Kâmil Uǧurbil (ORCID: https://orcid.org/0000-0002-8475-9334)
- Sarah R. Heilbronner (ORCID: https://orcid.org/0000-0003-0893-5364)
- Steve Jungst (ORCID: https://orcid.org/0000-0001-8374-7924)
- Christophe Lenglet (ORCID: https://orcid.org/0000-0003-4646-3185)
- Franco Pestilli (ORCID: https://orcid.org/0000-0002-2469-0494)
- Steen Moeller (ORCID: https://orcid.org/0000-0003-1698-7260)
- Benjamin C. Tendler (ORCID: https://orcid.org/0000-0003-2095-8665)
- Karla L. Miller (ORCID: https://orcid.org/0000-0002-2511-3189)
- Wenchuan Wu (ORCID: https://orcid.org/0000-0002-5020-5165)
Publication Details
- Journal
- Proceedings on CD-ROM - International Society for Magnetic Resonance in Medicine. Scientific Meeting and Exhibition/Proceedings of the International Society for Magnetic Resonance in Medicine, Scientific Meeting and Exhibition
- Published
- 2025-09-16
- DOI
- https://doi.org/10.58530/2025/4069
- Primary Topic
- Advanced Neuroimaging Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00