Micron-resolution fiber mapping in brain histology independent of sample preparation
Motivation: Mapping brain's intricate fiber network is a key quest in neuroscience. However, diffusion MRI is expensive, slow, and probes thousands of fibers/voxel, while micron-resolution methods typically do not resolve crossings, probe limited filed-of-view, or need special sample preparations. Goal(s): To map fibers in brain sections from any sample preparation with micron-resolution. Approach: We use computational scattered light imaging (ComSLI) with a simple rotating light and camera setup on new or archived brain histology sections. Results: We generate micron-resolution fiber maps from histology sections of healthy and diseased human and animal brains, prepared with different protocols, providing ground-truth information for diffusion MRI fiber mapping. Impact: Using scattered light, we generate micrometer maps of brain fiber orientations in animal and human histology sections, and compare to diffusion MRI. This enables time- and cost-effective studies of brain micro-architecture on new and archived sections from any sample preparation.
Authors
- Michael Zeineh (ORCID: https://orcid.org/0000-0002-6940-9096)
- Marios Georgiadis (ORCID: https://orcid.org/0000-0003-0733-4559)
- Katrin Amunts (ORCID: https://orcid.org/0000-0001-5828-0867)
- Jeffrey Nirschl (ORCID: https://orcid.org/0000-0001-6857-341X)
- Hamed Abbasi (ORCID: https://orcid.org/0000-0003-0655-7187)
- Markus Axer (ORCID: https://orcid.org/0000-0001-5871-9331)
- Sjors A. Koppes (ORCID: https://orcid.org/0000-0002-8833-531X)
- Congyu Liao (ORCID: https://orcid.org/0000-0003-2270-276X)
- Mackenzie Carlson (ORCID: https://orcid.org/0000-0002-0857-3116)
- Stijn Keereweer (ORCID: https://orcid.org/0000-0003-4665-6868)
- Miriam Menzel (ORCID: https://orcid.org/0000-0002-6042-7490)
- Hossein Moein Taghavi (ORCID: https://orcid.org/0000-0002-8585-1634)
- Raymond A. Sobel (ORCID: https://orcid.org/0000-0002-0477-9002)
- Kawin Setsompop (ORCID: https://orcid.org/0000-0003-0455-7634)
- Franca auf der Heiden (ORCID: https://orcid.org/0009-0002-7940-363X)
- Loes Ettema
- Moe Wakatsuki
- Andy Liu
- Michail Doukas (ORCID: https://orcid.org/0000-0002-8611-840X)
- William Ho
Publication Details
- Journal
- Proceedings on CD-ROM - International Society for Magnetic Resonance in Medicine. Scientific Meeting and Exhibition/Proceedings of the International Society for Magnetic Resonance in Medicine, Scientific Meeting and Exhibition
- Published
- 2025-09-16
- DOI
- https://doi.org/10.58530/2025/0717
- Primary Topic
- Advanced Neuroimaging Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00