Brain metabolism profile predicts the upregulation of cold-stress proteins after hypothermia in murine hypoxia-ischemia

Motivation: To explore biomarkers for recognizing non-responders to hypothermia Goal(s): To test if the metabolic profile with hyperpolarized 13C MR spectroscopy after hypothermia predicts the upregulation of cold-stress proteins. Approach: Postnatal 10 mouse pups with hypoxia-ischmia (HI) were divided into HI with normothermia, HI with hypothermia and sham groups, and underwent HP-13C MRS scans. Brains were harvested for cold-stress proteins' measurements Results: Anaerobic metabolism was suppressed 4h and 20h after hypothermia and the reduction of Kpl and Lac/Pyr can predict the upregulation of cold stress protein, i.e. CIRP levels. Impact: The cold stress protein, i.e., CIRP levels could be used as one of the hypothermia-regulated targets to promote the TH effect on those who are not responsive to the hypothermia.