Preparation of Fe-Mn Co-doped 2D MXene Nanoprobes and Feasibility Study of Combining with CEST for Multimodal Imaging

Motivation: Novel magnetic nanoparticles offer promise in MRI with low toxicity and T1 enhancement, particularly for detecting changes in the tumor microenvironment. Goal(s): To develop a Ti3C2-MXene-based dual-mode contrast agent doped with iron and manganese for enhanced T1/T2 imaging and investigate its multi-modal imaging capabilities with CEST agents. Approach: Synthesized single-layer Ti3C2 was doped with Fe3O4 and MnO2, then modified with carboxymethyl chitosan. Various techniques characterized the molecular probe, which was tested with CEST agents for MRI and photothermal effects. Results: The probe, combined with Ioversol, suppressed CEST signals at 0.5 mg/mL, allowing clear dual-mode imaging, and demonstrated excellent photothermal efficiency in vitro. Impact: This approach enables simultaneous imaging of dual contrast agents with distinct concentration ranges, with CEST signal variations reflecting probe concentration. The molecular probes also show potential for effective photothermal therapy, advancing multi-functional cancer diagnostic and treatment options.