Evaluation of Physiological Characteristics of in vivo AGN Probe Using Multimodal Imaging

Motivation: Monitoring the enzymatic activity of APN in tumors non-invasively can improve cancer diagnostics and therapy. A hyperpolarized AGN probe is a key biomarker for APN Goal(s): This study aimed to validate the physiological characteristics of AGN probes in vivo Approach: We used hyperpolarized 13C MRS and EPSI to measure APN activity in tumors with varying APN expression. DCE-MRI, DWI, and EPR imaging to evaluate several tumor physiological parameters Results: The AGN probe distinguished high and low APN-expressing tumors, even in low permeability and hypoxic regions, showing promise for personalized cancer treatment Impact: The hyperpolarized AGN probe provides a novel, non-invasive method for real-time monitoring of APN activity in tumors, even in hypoxic and low-perfusion regions, thus providing therapeutic strategies and personalized cancer treatment